JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:06Introduction
- 00:51Cardiac Puncture and Dissection of Thymus
- 02:52Isolation of Antigen Presenting Cells (APCs) from Thymus
- 06:24Activation of OVA-specific T cells by Thymic APCs
- 12:55Adoptive Transfer of OVA-specific T cells
- 19:27Challenging with OVA in the Ear
- 21:02Measurment of Inflammation in the Ear
- 21:27Conclusion
Verzögerte Überempfindlichkeitsreaktionen (DTH) ist eine entzündliche Reaktion durch CCR7-Effektor-Memory-T (TEM)-Lymphozyten vermittelt. Hier zeigen wir, wie um Antigen-spezifische TEM-Zellen zu aktivieren, bewegen Adoptiveltern DTH in Lewis-Ratten und überwachen die entzündliche Reaktion.
Tags
InductionMonitoringAdoptive Delayed-type HypersensitivityRatsDTHInflammatory ReactionCCR7-Effector Memory T LymphocytesAntigenImmune SystemImmunosuppressantsCutaneous DTHAdoptive TransferActive ImmunizationIntradermal ChallengeOvalbuminTuberculinTetanus ToxoidKeyhole Limpet HemocyaninAutoantigenMyelin Basic Protein (MBP)Experimental Autoimmune Encephalomyelitis (EAE)Lewis RatsStimulationOvalbumin-specific T CellsIntraperitoneally InjectionEquilibrate In VivoPinnaSaline3-72 Hours Later
