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视频率扫描共聚焦显微镜和内窥镜
JoVE 신문
생체공학
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JoVE 신문 생체공학
Video-rate Scanning Confocal Microscopy and Microendoscopy
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视频率扫描共聚焦显微镜和内窥镜

DOI:
10.3791/3252-v

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14:10 min

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October 20, 2011

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,
1Program in Biophysics,Harvard University , 2Division of Health Sciences and Technology,Harvard-MIT, 3Wellman Center for Photomedicine, Massachusetts General Hospital,Harvard Medical School

Chapters

  • 00:05Title
  • 01:01Setting Up the Resonant Galvanometric Mirror and Relay Optics
  • 04:56Setting Up the Second Scanning Mirror and Rotating the Microscope
  • 06:37Setting Up the Scan, Tube, and Objective Lenses
  • 08:17Setting Up and Aligning the Confocal Pinhole and Detector
  • 12:38The Assembled System and Representative Images
  • 13:43Conclusion

Summary

자동 번역

자동 번역

定制的，实时的共焦扫描成像系统的完整的建筑描述。该系统，该系统可以很容易地用率视频显微镜和内窥镜，使成像的几何形状和应用程序使用标准的商用共焦系统成本的一小部分，而不是访问数组。

Tags

Confocal MicroscopyHigh-resolution ImagingOptical SectioningConfocal MicroendoscopyThree-dimensional ResolutionFluorescenceWide-field MicroscopeConfocal ApertureScanning MirrorsPrimary Dichroic Mirror

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