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Chapters
- 00:05Title
- 01:21In vivo Labeling DT40 Cells
- 02:04Cell Lysis and DNA Spreading
- 02:53Immunofluorescence Staining
- 04:31Image Acquisition and Data Analysis
- 05:24Replication Structures
- 06:54Conclusion
DT40, un sistema modello vertebrato genetico, fornisce un potente strumento per analizzare la funzione delle proteine. Qui si descrive un metodo semplice che permette l'analisi qualitativa dei parametri che influenzano la sintesi del DNA durante la fase S in DT40 cellule a livello di singola molecola.
Tags
DNA ReplicationVisualizationVertebrate Model SystemDT40DNA Fiber TechniqueReplication Fork StabilityGenome DuplicationEndogenous DNA DamageExogenous DNA DamageGenomic InstabilityTumor DevelopmentFluorescence MicroscopyAvian B-cell Line DT40Protein FunctionReverse GeneticsDNA Replication And Genome StabilityReplication Fork DynamicsDNA Synthesis RateQualitative AnalysisDNA Fiber TechniqueHalogenated Nucleotides
