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Chapters
- 00:01Title
- 00:09Introduction
- 02:08Preparing Reagents
- 03:00Incubating Human Blood with RosetteSep NK Enrichment Cocktail
- 05:15Isolating NK Cells after Histopaque Density Gradient Centrifugation
- 11:07Conclusion
Natural killer cells are a small population of lymphocytes. Here we show how to isolate these cells from human blood by negative selection, using a kit from StemCell Technologies. The cells obtained are viable and untouched by antibodies, and therefore ready to be used for a number of procedures.
