This content is Free Access.
Chapters
- 00:05Title
- 01:24Preparing the Perfusion and Imaging Setup
- 03:23Langendorff Perfusion of the Isolated Heart
- 05:25Loading Voltage and Calcium Sensitive Dyes
- 06:38Optical Imaging and Data Analysis
- 07:47Conclusion
Dit document beschrijft de dissectie procedure, instrumentale setup, en de experimentele omstandigheden tijdens de optische in kaart brengen van de transmembraan potentiaal (Vm) en intracellulair calcium van voorbijgaande aard (CAT) in intacte geïsoleerde Langendorff doorbloed muis harten.
Tags
Optical MappingAction PotentialsCalcium TransientsMouse HeartCardiovascular StudiesFluorescence ImagingGenetic EngineeringPhysiological PhenotypingTransmembrane Potential (Vm)Excitation-contraction CouplingLangendorff Perfused Mouse HeartsHeart FailureArrhythmiasMetabolic DiseaseHeart DiseasesActivation VisualizationConduction VelocityAction Potential DurationInstrumentation SetupExperimental ConditionsSpatio-temporal ResolutionCMOS Imaging Technology
