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Chapters
- 00:05Title
- 01:24Preparing the Perfusion and Imaging Setup
- 03:23Langendorff Perfusion of the Isolated Heart
- 05:25Loading Voltage and Calcium Sensitive Dyes
- 06:38Optical Imaging and Data Analysis
- 07:47Conclusion
Ce document détaille la procédure de dissection, la configuration instrumentale, et des conditions expérimentales lors de la cartographie optique de potentiel transmembranaire (Vm) et de calcium intracellulaire transitoire (CAT) dans Langendorff isolés perfusés intacte cœur de la souris.
Tags
Optical MappingAction PotentialsCalcium TransientsMouse HeartCardiovascular StudiesFluorescence ImagingGenetic EngineeringPhysiological PhenotypingTransmembrane Potential (Vm)Excitation-contraction CouplingLangendorff Perfused Mouse HeartsHeart FailureArrhythmiasMetabolic DiseaseHeart DiseasesActivation VisualizationConduction VelocityAction Potential DurationInstrumentation SetupExperimental ConditionsSpatio-temporal ResolutionCMOS Imaging Technology
