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RNA的分离绿脓杆菌殖民小鼠胃肠道
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
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RNA的分离绿脓杆菌殖民小鼠胃肠道

DOI:
10.3791/3293-v

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08:52 min

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September 28, 2011

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, , ,
1Department of Pediatrics,University of Texas Southwestern Medical Center , 2Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital,Harvard Medical School, 3Department of Pediatrics and Microbiology,University of Texas Southwestern Medical Center

Chapters

  • 00:05Title
  • 01:21Harvesting Murine Luminal Contents
  • 02:44Bacterial RNA Isolation
  • 06:03DNAse Treatment
  • 07:48Representative Agilent Biolanalyzer Analysis of Murine Cecal Content-extracted Bacterial RNA
  • 08:35Conclusion

Summary

자동 번역

자동 번역

RNA提取的一种可靠的方法<em>绿脓杆菌</em从小鼠cecums恢复描述。回收的RNA是足够的数量和质量，为后续的qPCR，转录分析和RNA序列的实验。这种技术可以适用于RNA等肠道微生物的隔离。

Tags

RNA IsolationPseudomonas AeruginosaGastrointestinal TractColonizationImmune SystemsLeukemiaBurn WoundsOrgan TransplantsBloodstream InfectionsTranscription ProfilingMRNAGene ExpressionImmune StatusIsolationHigh Quality MRNAEnzymesDebrisFood ResiduesParticulate MatterGI ColonizationNeutropenia-induced DisseminationMice Euthanization

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