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Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
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Chapters
- 00:05Title
- 01:50Introduction
- 02:42Preparation of Chemoattractant in Agarose Gel
- 04:30Preparation of Cremaster Muscle for Intravital Microscopy
- 10:14Cell Tracking Using ImageJ
- 12:18Analysis of Neutrophil Recruitment Parameters
We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.
Tags
TrackingNeutrophilIntraluminal CrawlingTransendothelial MigrationChemotaxisIntravital Video MicroscopyLeukocytesInflammationPostcapillary VenulesLuminal SurfaceRolling LeukocytesEndotheliumFirm AdhesionChemokineChemoattractantsVenular SurfaceIntraluminal CrawlingTransmigrationChemoattractant GradientBrightfield Intravital MicroscopyMouse Cremaster MuscleNeutrophil RecruitmentAgarose Gel
