This content is Free Access.
Chapters
- 00:05Title
- 01:15Isolating Cardiomyocyte
- 03:37Isolating Single Cells
- 04:08Single Cell qPCR
- 05:26Whole Transcriptome Amplification and Microarray of Single Cells
- 07:00Quality Control Results
- 07:56Conclusion
Einzel-Zell-Expression Profiling ermöglicht die detaillierte Genexpressionsanalyse einzelner Zellen. Wir beschreiben Methoden zur Isolierung von Kardiomyozyten, und die Vorbereitung der daraus resultierenden Lysate entweder für ganze Transkriptom-Mikroarray oder qPCR der spezifischen Ziele.
Tags
Single CellTranscriptional ProfilingAdult MouseCardiomyocytesGene ExpressionHomogenatesStochastic VariationPure Cardiomyocyte PopulationsCollagenase PerfusionMicroarraysWhole TranscriptomeQPCRNanofluidic ArraysGene Expression ProfilesEvaluation MetricsVariance Between CellsRobust AmplificationDouble Stranded CDNANimbleGen ArraysReverse Transcriptase – Specific Target Amplification (RT-STA)Nanofluidic PCRVariability Of Expression
