Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

DOI:

10.3791/3303-v

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08:40 min

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April 06, 2012

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Matthew J. Coussens*¹, Kevin Forbes*¹, Carol Kreader*¹, Jack Sago, Carrie Cupp, John Swarthout

¹Sigma-Aldrich

Chapters

00:05Title
01:39Transfection with Target ID Library and Selection for Stable Cell Lines
03:59Selection Reagent Kill Curves
04:52Transfection of Library Cells with miRNA Expression Constructs and Selection for Stable Cell Lines and miRNA Targets
06:53Library Screen for miR-373 Targets
08:19Conclusion

Summary

자동 번역

The Target ID Library is a plasmid-based, genome-wide collection of cloned cDNA used to identify miRNA targets. Here we demonstrate its use and application.

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

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Chapters

Summary

자동 번역

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