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丰富的少突胶质细胞培养和Myelinating产后小鼠组织联合培养的少突胶质细胞/神经元的推导

JoVE 신문
신경과학
Author Produced

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JoVE 신문 신경과학

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

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丰富的少突胶质细胞培养和Myelinating产后小鼠组织联合培养的少突胶质细胞/神经元的推导

DOI:

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18:17 min

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August 21, 2011

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Ryan W. O’Meara², Scott D. Ryan, Holly Colognato, Rashmi Kothary^2,4

¹Regenerative Medicine Program,Ottawa Hospital Research Institute, ²Department of Cellular and Molecular Medicine,University of Ottawa , ³Department of Pharmacological Sciences,Stony Brook University, ⁴Department of Medicine,University of Ottawa

Chapters

00:01Title
00:29개요
01:27Generation of Mixed Glial Cultures and Purification of Oligodendrocyte Precursor Cells
10:50Generation of Neuron Cultures for Production of Myelinating Co-cultures
16:06Representative Results
17:35Conclusion

Summary

자동 번역

本文介绍的方法，从中获得丰富的人口在原代培养的小鼠少突胶质前体细胞（OPCS），它分化为成熟的少突胶质细胞（OLS）。此外，本报告介绍的技术生产小鼠myelinating播种到小鼠背根神经节（DRGNs）突起床鼠标OPCs的合作文化。

Tags

Enriched Oligodendrocyte Cultures Oligodendrocyte/Neuron Myelinating Co-cultures Molecular Mechanisms OL Development Demyelinating Diseases Multiple Sclerosis (MS)Primary Cell Culture Models Rats Mice Extraction Of OPCs Neurosphere Derivation Differential Adhesion Purification Immunopurification McCarthy & De Vellis Method

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