JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:37Preparation of Agar-embedded Retinal Slices
- 04:21Electrophysiological Recordings and Ca2+ Imaging
- 07:23Results: Identification and Ca2+ Imaging of Bipolar Cell Synaptic Terminals in Retinal Slices
- 08:49Conclusion
Her beskriver vi en protokoll for utarbeidelse av agar-embedded retinal skiver som er egnet for elektrofysiologi og Ca2 + bildebehandling. Denne metoden gjør det mulig å studere bånd-type synapser i retinal microcircuits bruke direkte patch-clamp opptak av single presynaptiske nerveterminaler.
Tags
Patch-clamp Capacitance MeasurementsCa2+ ImagingSingle Nerve TerminalsRetinal SlicesVisual StimuliDynamic RangeLight IntensitiesFrequency ChangesSpecialized NeuronsVertebrate RetinaPhotoreceptorsBipolar CellsRibbon-type Active ZonesNeurotransmitter ReleaseON-type Mixed Bipolar Cell (Mb) TerminalsGoldfish RetinaRod And Cone Photoreceptor InputRibbon-type SynapsesSize Of TerminalsLateral And Reciprocal Synaptic ConnectionsAmacrine Cell DendritesPatch-clamp TechniquePresynaptic Ca2+ CurrentsMembrane Capacitance ChangesReciprocal Synaptic Feedback InhibitionGABAA ReceptorsGABAC ReceptorsExocytosisShort-term Plasticity Of Excitatory Neurotransmitter ReleaseInhibitory Neurotransmitter Release From Amacrine Cells
