JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:04EVT Cells and Collagen Bead Incubation
- 02:13Loading the Rotating Cell Culture System (RCCS)
- 03:41Changing the Media
- 04:37Collecting Propagated Cells
- 05:39SGHPL-4 Trophoblast Cells in a 3D Milieu
- 06:30Conclusion
Traditionel, todimensionale cellekultur teknikker ofte resultere i ændrede egenskaber med hensyn til differentiering markører, cytokiner og vækstfaktorer. Tre-dimensionel cellekultur i den roterende cellekultur-system (RCCS) genopretter udtryk for mange af disse faktorer, som vist her med en extravillous trofoblasten cellelinje.
Tags
Rotating Cell Culture SystemsHuman Cell CultureHuman Trophoblast CellsModelPrimary CulturesExplant CulturesTrophoblast Cell LinesInvasion Of Uterine WallRemodeling Of Uterine Spiral ArteriesExtravillous Trophoblast CellsSuccessful Establishment Of PregnancyIn Vitro Cell Culture ModelsRotating Cell Culture System (RCCS)Morphological PropertiesPhenotypic PropertiesFunctional PropertiesEVT-like Cell LinesDifferentiation Of EVTsGene ExpressionMatrix Metalloproteinases (MMPs)Trophoblast DifferentiationSaint Georges Hospital Placental Cell Line-4 (SGHPL-4)RCCS Culture VesselThree-dimensional Environment
