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在神经退行性疾病的高内涵筛选
JoVE 신문
의학
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JoVE 신문 의학
High Content Screening in Neurodegenerative Diseases
DOI:

13:32 min

January 06, 2012

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Chapters

  • 00:05Title
  • 01:20Automated Adherent Cell Culture Process
  • 03:31shRNA Virus Production and Plating onto Assay Plates
  • 05:44Lentiviral Transduction and Neuronal Differentiation of SH-SY5Y Cells
  • 06:57High Content Image Acquisition and Image Analysis
  • 09:27Representative Results of High Throughput Content Screening
  • 13:09Conclusion

Summary

자동 번역

我们描述了一种方法,自动细胞培养相结合的高含量的成像,可视化和量化多个细胞过程和结构,在高通量的方式。这种方法可以帮助在进一步的基因组功能注释以及确定疾病基因网络和潜在的药物靶点。

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