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Chapters
- 00:05Title
- 01:39Preparation of the Electrostatic Chamber
- 02:54Neural Progenitor Cell Isolation
- 04:09Application of the Electric Field to the Electrostatic Chamber
- 07:18Preparation of the Organotypic Spinal Cord Slice
- 08:40Injection of Hoechst 33342 Labeled Neural Progenitor Cells into the Organotypic Spinal Cord Slice
- 09:38Results: Neural Progenitor Cells Show Directed Migration in the Electric Field and the Organotypic Spinal Cord Slice
- 10:46Conclusion
Dit protocol laat zien methoden die worden gebruikt om 2D-en 3D-omgevingen tot stand in de speciaal ontworpen electrotactic kabinet, kan volgen cellen<em> In vivo / ex vivo</em> Met behulp van time-lapse opname op de enkele cel niveau, met het oog op galvanotaxis / electrotaxis en andere cellulaire reacties te onderzoeken om de huidige (DC) elektrische velden (EFS) te sturen.
