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Pouring and Running a Protein Gel by reusing Commercial Cassettes
JoVE 신문
생물학
Author Produced
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JoVE 신문 생물학
Pouring and Running a Protein Gel by reusing Commercial Cassettes
DOI:

11:03 min

February 12, 2012

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Chapters

  • 00:05Title
  • 00:06Introduction
  • 00:48Preparing the Cassettes
  • 01:56Pouring the Resolving Gel
  • 04:01Pouring the Stacking Gel
  • 06:00Storing the Gel
  • 06:32Preparing the Samples
  • 07:35Running the Gel
  • 09:13Staining the Gel
  • 10:17Cleaning the Cassettes
  • 10:35Conclusion

Summary

자동 번역

Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.

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