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γHV68感染小鼠的测量
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Measurement of γHV68 Infection in Mice
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γHV68感染小鼠的测量

DOI:
10.3791/3472-v

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13:22 min

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November 22, 2011

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, , , , ,
1Department of Molecular Microbiology and Immunology,University of Southern California, Los Angeles

Chapters

  • 00:05Title
  • 01:26Intranasal Infection of Mice with γHV68
  • 02:26Plaque Assay: Viral Titer Determination in Acutely Infected Lungs
  • 06:50Infectious Center Assay: Virus Load in Latently Infected Splenocytes
  • 10:47Representative Assay Results
  • 13:01Conclusion

Summary

자동 번역

자동 번역

γ-疱疹病毒（γ- HVS）在其主机建立终身续保。感染小鼠γ- HV68提供了一种基因听话<em>在体内</em>模型为表征的生命周期/γHVs发病机制。这个协议描述γHV68感染的检测和定量菌斑形成，传染病中心，qPCR实验感染后的急性和潜伏阶段。

Tags

MeasurementγHV68InfectionMiceγ-herpesvirusesLatent InfectionsLymphoid CellsHost RangeEBVKSHVPathogenic StudiesMurine γ-herpesvirus 68Genetic SimilaritiesBiological SimilaritiesViral InfectionViral LifecyclePathogenesisIntranasal InoculationAcute ViremiaLatent InfectionSplenocytesReactivationPlaque AssayInfectious Virus TiterLung HomogenatesVero Cell MonolayersPost-intranasal InfectionAcute InfectionLatent Infection Spleen

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