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鼠标感觉神经支配的皮肤和四肢骨骼纤维组织的制备及免疫染色

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Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones

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鼠标感觉神经支配的皮肤和四肢骨骼纤维组织的制备及免疫染色

DOI:

•

10:25 min

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January 26, 2012

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Andrew J. Shepherd, Durga P. Mohapatra²

¹Department of Pharmacology,The University of Iowa, ²Department of Anesthesia,Roy J. and Lucille A. Carver College of Medicine, The University of Iowa

Chapters

00:05Title
01:42Tissue Dissection, Post-fixation, and Sectioning
05:37Immunostaining: Floating Section Staining for Plantar Punch Tissue
07:17Immunostaining: On-slide Staining for Limb Bone Sections
08:38Epifluorescence and Confocal Imaging of Immunostained Plantar Punch and Bone Tissue

Summary

자동 번역

末梢感觉神经纤维亚型的免疫细胞化学鉴定（和蛋白表达其中的检测）底层外围感觉的分子机制的理解是关键。在这里，我们描述外设/内脏组织样本，特定的免疫外周感觉神经纤维，如皮肤和四肢骨骼的制备方法。

Tags

Immunostaining Mouse Sensory Nerve Fibers Skin Innervation Limb Bones Innervation Sensory Perception Receptors Ion Channel Proteins Electrophysiological Methods Visceral Organs Innervation Bone Innervation Non-excitable Proteins Immunostaining Procedures Sensory Ganglia Protein Expression Studies

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