Journal
/
생물학
/
Analyse af Cell Cycle Position i pattedyrceller
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Analysis of Cell Cycle Position in Mammalian Cells

Analyse af Cell Cycle Position i pattedyrceller

DOI:
10.3791/3491-v

•

12:19 min

•

January 21, 2012

•

, ,
1Department of Biochemistry, Schulich School of Medicine and Dentistry,University of Western Ontario, 2London Regional Cancer Program, Children’s Health Research Institute, and Department of Biochemistry, Schulich School of Medicine and Dentistry,University of Western Ontario

Chapters

  • 00:05Title
  • 01:19Labeling and Fixing of Cells
  • 02:30Denaturing and Staining of Brdu and DNA
  • 04:11Flow Cytometry and Data Analysis
  • 06:10Alternate Protocol for Cell Cycle Analysis in a Mixed Population of Cells
  • 07:35Cell Cycle Distribution Staining Can Be Used for a Variety of Experimental Purposes
  • 11:47Conclusion

Summary

자동 번역

자동 번역

Bestemmelse af cellecyklus position af en population af celler, eller forstå, hvordan signaler påvirker spredningen umiddelbart kan måles ved flowcytometri hjælp af denne protokol. Vi rapporterer en enkel eksperimenterende tilgang til farvning af celler og kvantificere deres position i cellecyklus.

Tags

Cell Cycle PositionMammalian CellsCell ProliferationCell Cycle PhasesDNA SynthesisGrowth RegulationPropidium IodideFlow CytometryRadioactive ThymidineBromodeoxyuridineFluorodeoxyuridineBrdU Staining

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/3491?article_type=v

Long-term Imaging Mammalian Cells using Wide-Field Microscopy
check_url/kr/3491?article_type=v

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
check_url/kr/3491?article_type=v

Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells
check_url/kr/3491?article_type=v

MISSION esiRNA for RNAi Screening in Mammalian Cells
check_url/kr/3491?article_type=v

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
check_url/kr/3491?article_type=v

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
check_url/kr/3491?article_type=v

Ekspression Analyse af mammale Linker-histon Undertyper
check_url/kr/3491?article_type=v

Visualisering af endoplasmatiske reticulum lokaliserede mRNA'er i pattedyrceller
check_url/kr/3491?article_type=v

Induktion og analyse af Epithelial til Mesenchymale Transition
check_url/kr/3491?article_type=v

Måling af hæmoglobinsyntese Niveauer i pattedyrceller

Read Article