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Chapters
- 00:05Title
- 01:26Tissue Preparation
- 03:04Preparation of Beads for Pull-down
- 04:04Protein Binding to CaM-sepharose and Elution
- 06:04Representative Pull-down Results
- 07:02Conclusion
Calmoduline (CaM) pull-down test est un moyen efficace pour étudier l'interaction de la CAM avec diverses protéines. Cette méthode utilise la CaM-sépharose perles pour une analyse efficace et spécifique des protéines liant la CaM. Cela fournit un outil important d'explorer de signalisation CAM dans la fonction cellulaire.
Tags
Calmodulin-binding ProteinsCalcium RegulationCellular FunctionCa2+ SignalingCalmodulin (CaM)ApoptosisMetabolismSmooth Muscle ContractionSynaptic PlasticityNerve GrowthInflammationImmune ResponseConformation Of CaMApoCaMTarget ProteinsIQ-domainNeuromodulinNeurogranin (Ng)MyosinsPresynaptic FunctionPostsynaptic FunctionMuscle ContractionCa2+-CaM BindingActivation Of ProteinsMyosin Light Chain KinaseCa2+/CaM-dependent Kinases (CaMKs)CalcineurinSpectrin KinaseDownstream Effects
