Journal
/
면역학 및 감염병학
/
Intravital Imaging of the Mouse Thymus mittels 2-Photonen-Mikroskopie
JoVE 신문
면역학 및 감염병학
Author Produced
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
Please note that all translations are automatically generated. Click here for the English version.

Intravital Imaging of the Mouse Thymus mittels 2-Photonen-Mikroskopie

DOI:
10.3791/3504-v

•

10:08 min

•

January 07, 2012

•

, ,
1Laboratory of Immune Regulation,Instituto Gulbenkian de Ciência

Chapters

  • 00:01Title
  • 00:05Introduction
  • 01:15Animal preparation: Irradiation and bone marrow transfer
  • 03:16Animal preparation: Thymic transplantation
  • 04:58Animal preparation: Surgery to exposure transplanted thymus
  • 06:53Two-photon image acquisition
  • 08:35Results

Summary

자동 번역

자동 번역

We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.

Tags

Intravital ImagingMouse Thymus2-photon MicroscopyImage AcquisitionOrgansStereotactic ToolsSurgical ProceduresCellular BehaviorsLive AnimalsEx Vivo Imaging AcquisitionSurgical ProcedureOrgan HolderFoxp3+ CellsRegulatory T CellsNaturally-occurring Tregs (nTregs)Peripherally-converted Tregs (pTregs)In Vivo InteractionsPhenotype And PhysiologyNude Mice

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/3504?article_type=v

Intravitalmikroskopie der Leistenbruch Lymph Node
check_url/kr/3504?article_type=v

Direkte Beobachtung der Phagozytose und NET-Bildung durch Neutrophile im infizierten Lungengewebe mittels 2-Photonen-Mikroskopie
check_url/kr/3504?article_type=v

Intravitalmikroskopie der Milz: Quantitative Analyse von Parasite Mobility und die Durchblutung
check_url/kr/3504?article_type=v

Bildgebung des dem Maus-Kniekehlen-Lymphknoten
check_url/kr/3504?article_type=v

Hochaufgelöste Intravitalmikroskopie Striped-Beleuchtung von Keimzentren
check_url/kr/3504?article_type=v

Intravitalmikroskopie Imaging der Leber nach<em> Leishmania</em> Infection: Eine Bewertung von Leber Hämodynamik
check_url/kr/3504?article_type=v

Langzeitintravital Multiphotonen Mikroskopie Imaging von Immunzellen in gesunden und kranken Leber Mit CXCR6.Gfp Reporter Mäuse
check_url/kr/3504?article_type=v

Analyse von<em> Yersinia enterocolitica</em> Effektor Translokation in Wirtszellen unter Verwendung Beta-Lactamase-Effektor-Fusionen
check_url/kr/3504?article_type=v

Abbilden Neutrophilen und Monozyten in Mesenterialvenen von Intravitalmikroskopie an narkotisierten Ratten in Echtzeit
check_url/kr/3504?article_type=v

Intravital Imaging von Neutrophilen Priming Mit IL-1β Promoter-driven DsRed Reporter Mäuse

Read Article