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Chapters
- 00:05Title
- 01:04Retroviral Labeling of Cells With Fluorescent Proteins
- 02:45Inverse Matrigel Invasion Assay
- 04:16Staining and Visualization
- 06:07Contributions of Candidate Proteins to Collective Invasion
- 06:50Conclusion
Models of tumor cell invasion into three-dimensional extracellular matrix better reflect the in vivo situation than two-dimensional motility assays. Using matrix invasion assays combined with confocal imaging of fluorescently-labeled cells, detailed information on invasion modes and the distinct contributions of leading versus following cells can be obtained.
Tags
ModelingImaging3-dimensionalCollective Cell InvasionCancer MalignancyInvasionMetastasisGliomaBreast CancerLung CancerMetastasis ProcessTumor CellsOrgan FailureMorbidityMortalityMolecular ProcessesProtein MediatorsDiagnosisTreatmentInvasion AssaysIn Vivo SituationTwo-dimensional Cell Motility AssaysExtracellular Matrix (ECM) Protein RemodelingTumor Cell InvasionIndividual Cells MovementCollective Invasion
