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Chapters
- 00:05Title
- 01:35Sample Preparation
- 02:42Isoelectric Focusing
- 04:17SDS Polyacrylamide Gel Electrophoresis
- 05:38In-gel Tryptic Digestion
- 07:38Results: Skeletal Muscle Protein
- 09:08Conclusion
A straight-forward set of methods to isolate and determine the identity of the most abundant proteins expressed in skeletal muscle. About 800 spots are discerned on a two-dimensional gel from 10 mg muscle; this allows for the determination of gender-specific protein expression. These methods will give equivalent results in most tissues.
Tags
Skeletal MuscleGender DimorphismProteomicsContractile ProteinsHypertrophyAtrophyRemodelingAdaptations To StressorsEccentric ContractionDamage To MuscleRepairDegradationResynthesisGlobal ProteomeExerciseProtein ExpressionDifferential Protein ExpressionAdaptations To Damage And RepairMale MuscleFemale MuscleTwo-dimensional Gel ElectrophoresisHigh Resolution Digital Imaging
