JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 03:11Germ Cell-transplantation Procedure
- 05:32Preparation of Donor Tissue for Xenotransplantation
- 06:57Castration of Recipient Mouse and Ectopic Xenografting
- 08:48Transplanted SSCs and Xenograft Development
- 09:54Conclusion
Protokolle für die Keimzelle der Transplantation und Hodengewebe Xenografting werden beschrieben. Germ-Transplantation führt zu Donor-derived Spermatogenese in den Empfängerländern Hoden und eine funktionelle Rekonstitution Assay für die Identifizierung von Spermatogonien-Stammzellen (SSC). Hodengewebe Xenografting reproduziert Hodenentwicklung und Spermatogenese verschiedener Spender Arten in Empfängermäuse.
Tags
Germ Cell TransplantationMiceDr. Ralph BrinsterUniversity Of PennsylvaniaMicroinjectionSeminiferous TubulesSpermatogenesisSperm ProductionDonor-derivedBacterial β-galactosidase GeneOffspringLumen Of The Seminiferous TubulesBasement MembraneSpermatogoniaSSCsStem Cell NichePutative Spermatogonial Stem CellsTransgenic AnimalsGenetic ManipulationSertoli Cell-germ Cell InteractionSSC Homing And ColonizationSelf-renewal And Differentiation
