JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:15Manufacturing Solid-state Nanopores in Silicon Nitride Membranes
- 03:52Wetting of the Solid-state Nanopore
- 06:18Monitoring Protein Adsorption
- 07:12Typical Solid-state Nanopores
- 08:15Conclusion
A method of using solid-state nanopores to monitor the non-specific adsorption of proteins onto an inorganic surface is described. The method employs the resistive-pulse principle, allowing for the adsorption to be probed in real-time and at the single-molecule level. Because the process of single protein adsorption is far from equilibrium, we propose the employment of parallel arrays of synthetic nanopores, enabling for the quantitative determination of the apparent first-order reaction rate constant of protein adsorption as well as and the Langmuir adsorption constant.
Tags
Solid-state NanoporesProtein AdsorptionSingle-molecule MeasurementsNucleic AcidsProtein UnfoldingBinding AffinityResistive-pulse TechniqueLabeling-free MeasurementsTransmembrane PotentialAnalyte PartitioningNanopore WallsMicrofluidic DevicesProtein Binding AssayNitride FilmsThin FilmsFunctionalized SurfacesDenaturing Conditions
