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Chapters
- 00:05Title
- 01:16Cell Culture
- 01:40Amino Acid Extraction
- 02:46Amino Acid Derivatization and GC-MS Conditions
- 04:07GC-MS Data Analysis
- 05:41Representative GC-MS Analysis
- 07:06Conclusion
13C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.
Tags
Metabolic Pathway ConfirmationMetabolic Pathway Discovery13C LabelingProteinogenic Amino Acids생화학Functional Genomics13C-assisted Metabolism AnalysisIsotopic LabelingAtom Transition PathsBiochemical NetworkTranscriptomicsProteomicsIsotopomer-assisted Analysis Of Metabolic PathwaysLabeled SubstratesMinimal MediumCarbon SourceMeasurement NoisesNon-labeled CarbonNutrient SupplementsLabeled Substrate ChoiceReaction StereochemistryIntermediate ProductsSingly Labeled Carbon SubstratesUniformly Labeled SubstratesAmino Acid Labeling PatternsGC-MS
