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Chapters
- 00:05Title
- 01:26Preparation of Plate for Image Acquisition
- 02:17Microscope Setup and Automated Image Acquisition
- 04:44Image Processing and Analysis of Migration: Calculation of Speed and Displacement
- 05:39Results: Random Migration Analysis and Videography
- 06:50Conclusion
This method allows monitoring of cells in real time and quantitative measurements of different cell migration parameters such as speed, displacement, and velocity. Unlike the traditional methods, this real time approach is not based on endpoint quantitative migration measurements; instead it allows monitoring and calculating different parameters continuously.
Tags
Quantitative AnalysisRandom MigrationCellsTime-lapse Video MicroscopyCell MigrationEmbryonic DevelopmentImmune System FunctionTumor InvasionChemotactic SignalIntrinsic CuesMotility MachineryLocal EnvironmentPolarizationProtrusionsBoyden Chamber Migration AssayChemotaxisEnd Point ResultIndividual Migration ParametersMorphological ChangesCancer Cell MigrationCancer Cell InvasionPlateletsPlatelet Function
