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随机使用时移视频显微镜细胞迁移的定量分析
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Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy
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随机使用时移视频显微镜细胞迁移的定量分析

DOI:
10.3791/3585-v

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07:27 min

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May 13, 2012

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, ,
1Department of Biochemistry and Molecular Biology,LSU School of Medicine, 2Department of Oral Biology,LSU School of Dentistry, 3Stanley S. Scott Cancer Center,LSU School of Medicine

Chapters

  • 00:05Title
  • 01:26Preparation of Plate for Image Acquisition
  • 02:17Microscope Setup and Automated Image Acquisition
  • 04:44Image Processing and Analysis of Migration: Calculation of Speed and Displacement
  • 05:39Results: Random Migration Analysis and Videography
  • 06:50Conclusion

Summary

자동 번역

자동 번역

这种方法允许在不同的细胞迁移参数，如速度，位移和速度的实时性和定量测量细胞的监测。实时方式与传统方法不同的是，这不是基于端点的定量迁移测量;相反，它允许不同的参数，不断监测和计算。

Tags

Quantitative AnalysisRandom MigrationCellsTime-lapse Video MicroscopyCell MigrationEmbryonic DevelopmentImmune System FunctionTumor InvasionChemotactic SignalIntrinsic CuesMotility MachineryLocal EnvironmentPolarizationProtrusionsBoyden Chamber Migration AssayChemotaxisEnd Point ResultIndividual Migration ParametersMorphological ChangesCancer Cell MigrationCancer Cell InvasionPlateletsPlatelet Function

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