JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:26Preparation of Plate for Image Acquisition
- 02:17Microscope Setup and Automated Image Acquisition
- 04:44Image Processing and Analysis of Migration: Calculation of Speed and Displacement
- 05:39Results: Random Migration Analysis and Videography
- 06:50Conclusion
Deze methode maakt het mogelijk controle van de cellen in real time en kwantitatieve metingen van verschillende celmigratie parameters, zoals snelheid, verplaatsing, en snelheid. In tegenstelling tot de traditionele methoden, is deze real-time benadering niet gebaseerd op eindpunt kwantitatieve migratie metingen, in plaats daarvan het laat de controle en het berekenen van verschillende parameters continu.
Tags
Quantitative AnalysisRandom MigrationCellsTime-lapse Video MicroscopyCell MigrationEmbryonic DevelopmentImmune System FunctionTumor InvasionChemotactic SignalIntrinsic CuesMotility MachineryLocal EnvironmentPolarizationProtrusionsBoyden Chamber Migration AssayChemotaxisEnd Point ResultIndividual Migration ParametersMorphological ChangesCancer Cell MigrationCancer Cell InvasionPlateletsPlatelet Function
