Journal
/
신경과학
/
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
JoVE 신문
신경과학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

DOI:
10.3791/3597-v

•

09:39 min

•

April 15, 2012

•

, , , ,
1Department of Biological Sciences,Florida Atlantic University, 2Department of Chemistry & Biochemistry,Florida Atlantic University

Chapters

  • 00:02Title
  • 00:48Electrophysiology rig set-up
  • 01:18Nanoinjection set-up
  • 03:14Drosophila melanogaster preparation
  • 03:58Paired nanoinjection/electrophysiology
  • 07:35Results

Summary

자동 번역

자동 번역

A rapid in vivo assay to test for neuromodulatory compounds using the Giant Fiber System (GFS) of Drosophila melanogaster is described. Nanoinjections in the head of the animal along with electrophysiological recordings of the GFS can reveal bioactivity of compounds on neurons or muscles.

Tags

NanoinjectionElectrophysiology AssayScreenBioactivityCompoundsDrosophila MelanogasterGiant Fiber SystemIn Vivo ActivityPharmacological AgentsModulatory EffectsNeuronal CircuitSmall MoleculesPeptidesMolecular TargetsGap JunctionsCholinergicNicotinic Acetylcholine ReceptorsNeuromuscular JunctionsGlutamatergicNanoliter QuantitiesIntracellular Recordings

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster

Whole Mount Preparation of the Adult Drosophila Ventral Nerve Cord for Giant Fiber Dye Injection

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster

High-resolution Quantification of Odor-guided Behavior in Drosophila melanogaster Using the Flywalk Paradigm

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster

Read Article