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Produktion af Tissue microarrays, Immunhistokemi farvning og digitalisering i den menneskelige protein Atlas
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생물학
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JoVE 신문 생물학
Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

Produktion af Tissue microarrays, Immunhistokemi farvning og digitalisering i den menneskelige protein Atlas

DOI:
10.3791/3620-v

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09:08 min

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May 31, 2012

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, , , ,
1Department of Immunology, Genetics and Pathology, Science for Life Laboratory,Uppsala University

Chapters

  • 00:05Title
  • 01:38Manual Tissue Microarray Production
  • 05:59How to Search for a Protein of Interest in the Human Protein Atlas
  • 07:06Representative Tissue Microarray Results
  • 08:35Conclusion

Summary

자동 번역

자동 번역

Væv microarrays muliggør en effektiv metode til at opnå samtidig information fra en lang række væv. Repræsentative dele af væv samles i en enkelt paraffin blok. Sektioner fra blokken anvendes til immunohistokemi og analyse af protein ekspressionsmønstre. Digital scanning genererer tilsvarende billeder til distribution af data.

Tags

Immunohistochemistry StainingDigitalizationHuman Protein AtlasHigh-throughput AnalysisProtein Expression PatternsTMA BlockCoresImmunostaining ProtocolExperimental VariabilityPatient CohortsConsecutive SectionsAntibodiesProtein ProfilesGlass Slides

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