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Het beoordelen van de lever metabole veranderingen tijdens Progressieve kolonisatie van Kiemvrije Mouse door 1 H NMR-spectroscopie
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
DOI:

07:54 min

December 15, 2011

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Chapters

  • 00:05Title
  • 01:12Colonization of Germ-free Animals and Sample Collection
  • 02:071H NMR Acquisition of Urine Microvolume
  • 03:25Collection of Liver Biopsy
  • 04:091H High Resolution Magic Angle Spinning (HR MAS) NMR of Liver Tissue Biopsy: Sample Preparation
  • 05:55Urinary and Hepatic Metabolic Profiling through 1H NMR and 1H HR MAS NMR Spectra
  • 07:32Conclusion

Summary

자동 번역

Een progressieve kolonisatie procedure wordt beschreven om nader te beoordelen de impact ervan op de host levermetabolisme. Colonization is gecontroleerd non invasief door het evalueren van de urinaire excretie van microbiële metabolieten door co-NMR-based metabool profiel, terwijl levermetabolisme wordt beoordeeld door de hoge resolutie Magic Angle Spinning (HR MAS) NMR profilering van intact biopsie.

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