JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:58Mammalian Cell Culture and Microinjection
- 04:32Fluorescent Microscopy
- 05:48Transmission Electron, and Correlative Light and Electron Microscopy
- 07:44Representative Imaging Results
- 08:48Conclusion
La técnica CLEM se ha adaptado para analizar la morfología ultraestructural de las membranas, orgánulos, y estructuras subcelulares afectados por moléculas microinyectados. Este método combina las poderosas técnicas de micromanipulación / microinyección, microscopía fluorescente confocal y microscopía electrónica para permitir a milímetro resolución nanométrica múltiples. Esta técnica es viable para una amplia variedad de aplicaciones.
Tags
Correlative Light And Electron MicroscopyCLEMVisualize Microinjected MoleculesEukaryotic Sub-cellular TargetsMembrane Bound CompartmentsBiochemical PolarityFluorescently Tagged MoleculesSubcellular CompartmentsCellular RegulationImaging TechniquesFluorescent MicroscopyConfocal MicroscopyResolutionElectron MicroscopySubcellular MorphologyDynamic ProcessesPrecisionSampleUltrafast Fluorescent ImagingHigh-resolution ImagingCell Biology
