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Chapters
- 00:05Title
- 01:58Part 1: Ventral Laparotomy
- 05:23Ventral Midline Incision
- 07:46Part 2: Transuterine Microinjection
- 08:32Common Embryo Orientations Observed by Transillumination of the Uterus
- 10:19Embryo Reorientation for Otocyst Injections
- 13:48Transuterine Microinjection
- 16:31Part 3: In vivo Electroporation
- 17:37Postnatal Selection of a Pup whose Otocyst was Injected at E12.5
- 19:47Prophylactic Analgesia
- 20:18Surgical Subject the Morning after Surgery
- 20:55Part 4: Representative Results
L'oreille interne de souris est un organe placode dérivé sensorielle dont le développement du programme est élaboré pendant la gestation. Nous définissons un<em> In utero</em> Technique de transfert de gène constitué de trois étapes: la souris laparotomie ventrale, la microinjection transutérine, et<em> In vivo</em> Électroporation. Nous utilisons la microscopie vidéo numérique pour démontrer les critiques de techniques expérimentales embryologiques.
Tags
Gene TransferDeveloping Mouse Inner EarIn Vivo ElectroporationSensory EpitheliaCristaeMaculaeOrgan Of CortiVestibular Hair CellsAuditory Hair CellsCell Fate SpecificationMorphogenesisGestationTransgenic EmbryosMolecular BasisGain-of-function StudiesLoss-of-function StudiesGene Misexpression StudiesVentral LaparotomyTransuterine Microinjection
