JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:02Preparation of Metal Raft Grids and Collagen Gels
- 03:03Preparing Keratinocytes for Differentiation
- 04:16Making the Raft Cultures
- 05:39Representative Organotypic Raft Culture Experiments
- 06:55Conclusion
An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.
Tags
Organotypic Raft CulturesPrimary Human KeratinocytesIn Vitro SystemEpithelial DifferentiationThree-dimensional CulturesKeratinocytesHuman Papillomavirus (HPV)Virus ProductionDysplastic LesionsEpithelial Cell CancersDrugs On Epithelial Cell DifferentiationAsselineau And PrunierasKopan Et Al.Collagen PlugsAir-liquid InterfaceStratify And DifferentiateFull Thickness EpitheliumDifferentiation-specific CytokeratinsHistological ExaminationMolecular TechniquesBiochemical Techniques
