Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Sample Preparation of Mycobacterium tuberculosis…
JoVE Journal
Immunology and Infection

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Immunology and Infection
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
 
Click here for the English version

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Article DOI: 10.3791/3673-v • 07:56 min • September 3rd, 2012
September 3rd, 2012 •
1, 1, 2, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, 2Department of Chemistry, University of Nebraska-Lincoln

챕터

요약
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

The metabolomic profile of Mycobacterium tuberculosis is determined after growth in broth cultures. Conditions can be varied to test the effects of nutritional supplements, oxidants, and anti-tuberculosis agents on the metabolic profile of this microorganism. Procedure for extract preparation is applicable for both 1D 1H and 2D 1H-13C NMR analyses.

Tags

Mycobacterium Tuberculosis Nuclear Magnetic Resonance Metabolomic Studies Sample Preparation Drug-resistant Strains Gene Function Gene Expression Analysis Global Patterns Metabolic Composition Small Molecular Weight Chemicals Target Identification Anti-tubercular Activity NMR Technology
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter