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Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
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Chapters
- 00:05Title
- 01:25In situ Hybridization
- 03:27Glycerol Mounting
- 05:30Dechorionation, Fixation, and Endochorion Disruption
- 08:04Peeling
- 09:44Representative In situ Results
- 10:36Conclusion
Temporal and spatial gene expression analyses have a crucial role in functional genomics. Whole-mount hybridization in situ is useful for determining the localization of transcripts within tissues and subcellular compartments. Here we outline a hybridization in situ protocol with modifications for specific target tissues in mosquitoes.
Tags
Hybridization In SituSalivary GlandsOvariesEmbryosVector MosquitoesPathogensTranscriptsGene RegulatorsMosquito-borne Disease TransmissionEgg DevelopmentGene Expression RegulationSpatial InformationAccumulation PatternsFemale Salivary GlandsOvaries And Embryos Subcellular CompartmentsMosquito Reproduction And DevelopmentRNA Probe PreparationFixation And Hybridization
