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Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:29Maize Kernal Bioassay
- 03:46Conidia Enumeration and Aflatoxin Quantification
- 05:46Fumonisin B1 Analysis
- 07:13Ergosterol Analysis
- 08:01Representative Fungal Growth Results
- 09:16Conclusion
The devastation of cereal crops by seed-infecting fungi has prompted numerous research efforts to better understand plant-pathogen interactions. To study seed-fungal interactions in a laboratory setting, we developed a robust method for the quantification of fungal reproduction, biomass, and mycotoxin contamination using kernel bioassays.
Tags
QuantificationFungal ColonizationSporogenesisMycotoxinsKernel BioassaysSeed-infecting FungiCereal ProductionMaizePathogen-induced LossesGrain IntegrityMycotoxin Seed ContaminationAflatoxinFumonisinAspergillus FlavusFusarium VerticillioidesMolecular Plant-pathogen InteractionsStandardized MethodQuantifying Biological ParametersCross-interpretationKernel AssaysQuantitative AnalysesFungal GrowthBiomassMycotoxin Contamination
