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Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea
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Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea

Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea

DOI:
10.3791/3731-v

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11:52 min

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September 14, 2012

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, , , ,
1Department of Pediatrics,David Geffen School of Medicine at UCLA

Chapters

  • 00:05Title
  • 01:45Dissection of Trachea
  • 02:46Cleaning and Cutting the Trachea
  • 03:45Enzyme Digestion and Processing into Single Cell Suspension
  • 06:42Staining for FACS and Sorting
  • 07:23Processing Sorted Cells for in vitro Culture and in vivo Model
  • 09:40Representative Data
  • 11:19Conclusion

Summary

자동 번역

자동 번역

Here we demonstrate our protocol for isolation of basal and submucosal gland duct cells from mouse tracheas. We also demonstrate the method of injecting stem cells into the dorsal mouse fat pad to create an in vivo model of submucosal gland regeneration.

Tags

IsolationBasal CellsSubmucosal Gland Duct CellsMouse TracheaLarge AirwaysRepair MechanismStem CellsIn Vitro Model SystemsIn Vivo Model SystemsMechanisms Of Repair And RegenerationAirway DiseasesCystic FibrosisAsthmaChronic Obstructive Pulmonary Disease

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