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Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
JoVE 신문
생물학
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JoVE 신문 생물학
Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
DOI:

17:38 min

April 27, 2012

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Chapters

  • 00:05Title
  • 02:16Tissue Preparation
  • 03:20Pre-hybridization Treatment of Tissue
  • 04:36Probe Hybridization
  • 07:34Visualization of the Radioactive Signal
  • 10:59Generating Darkfield Color Images
  • 11:38Results: mRNA Expression Patterns in Avian Brain
  • 16:56Conclusion

Summary

자동 번역

This protocol is successfully used to quantitatively detect levels and spatial patterns of mRNA expression in multiple tissue types across vertebrate species. The method can detect low abundance transcripts and allows processing of hundreds of slides simultaneously. We present this protocol using expression profiling of avian embryonic brain formation as an example.

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