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GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
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GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil

DOI:
10.3791/3767-v

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11:06 min

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May 19, 2012

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, ,
1DOE-Great Lakes Bioenergy Research Center,University of Wisconsin, Madison, 2Department of Soil Science,University of Wisconsin, Madison, 3Department of Soil and Water Science,University of Florida

Chapters

  • 00:05Title
  • 01:52Sample Preparation and Purification
  • 05:06Derivatization
  • 06:06Separation and Measurement
  • 08:11Representative Results
  • 09:55Conclusion

Summary

자동 번역

자동 번역

We describe a method protocol for the GC-based analysis of the aldonitrile acetate derivatives of glucosamine and muramic acid extracted from soil. For elucidation of the chemical mechanism, we also present a strategy to confirm the structure of the derivative and the ion fragments formed upon electron ionization.

Tags

GC-based DetectionAldononitrile Acetate DerivatizedGlucosamineMuramic AcidMicrobial Residue DeterminationSoil Microbial AnalysisBiomarker AnalysisGlucosamine And Muramic Acid AnalysisAnalytical MethodsDerivatizationGC-based AnalysisPrecisionSensitivityChromatographic SeparationSample Storage

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