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Chapters
- 00:00Title
- 00:39Introduction
- 02:36SU8 Master Fabrication
- 04:20PDMS Mold Fabrication
- 06:41Using PDMS Membrane
- 07:50Inserting C. elegans, Drosophila and Zebrafish Larvae
- 09:59Conclusions
A simple microfluidic device has been developed to perform anesthetic free in vivo imaging of C. elegans, intact Drosophila larvae and zebrafish larvae. The device utilizes a deformable PDMS membrane to immobilize these model organisms in order to perform time lapse imaging of numerous processes such as heart beat, cell division and sub-cellular neuronal transport. We demonstrate the use of this device and show examples of different types of data collected from different model systems.
