Journal
/
생물학
/
I Ovo Elektroporation i embryonisk Chick Retina
JoVE 신문
생물학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
In Ovo Electroporation in Embryonic Chick Retina

I Ovo Elektroporation i embryonisk Chick Retina

DOI:
10.3791/3792-v

•

06:15 min

•

February 05, 2012

•

, ,
1Department of Pharmacology,University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 2Department of Biomedical Engineering,Rutgers University

Chapters

  • 00:05Title
  • 00:56Egg Handling and Needle Preparation
  • 03:20Injection and Electroporation

Summary

자동 번역

자동 번역

Det overordnede mål for denne video er at vise, hvordan du udfører målrettet retinal injektion og<em> In ovo</em> Elektroporering af DNA / RNA konstrukter ind i kyllingens embryoniske retina i det Hamburger og Hamilton stadium 22-23, hvilket er omkring embryonisk dag 4 (E4). Denne teknik er meget nyttigt at undersøge genekspression, genregulering, og morfologiske forandringer i udviklingen af ​​chick nethinden.

Tags

Ovo ElectroporationEmbryonic Chick RetinaRetinal DevelopmentGene RegulationRecombinant DNA/RNA TechnologyReporter Gene AssayGene Over-expressionGene Knock DownTargeted Retinal InjectionHamburger And Hamilton Stage 22-23Embryonic Day 4 (E4)Green Fluorescent Protein (GFP)Subretinal SpaceRetinal Stem/progenitor CellsLate-born Neurons

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/3792?article_type=v

In Ovo Electroporations of HH Stage 10 Chicken Embryos
check_url/kr/3792?article_type=v

Method for Whole Mount Antibody Staining in Chick
check_url/kr/3792?article_type=v

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
check_url/kr/3792?article_type=v

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
check_url/kr/3792?article_type=v

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

check_url/kr/3792?article_type=v

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation
check_url/kr/3792?article_type=v

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
check_url/kr/3792?article_type=v

Single Cell Transfection in Chick Embryos
check_url/kr/3792?article_type=v

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
check_url/kr/3792?article_type=v

In vivo Elektroporation af Morpholinos i den voksne zebrafisk Retina

Read Article