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Chapters
- 00:05Title
- 01:28Infecting Cells with Retrovirus Expression Reprogramming Factors
- 03:31Isolation and Expansion of Induced Pluripotent Stem Cells
- 04:42Analysis of Pluripotent Markers by Immunofluorescence and Quantitative Real-time PCR
- 06:06Representative Cell-reprogramming Results
- 08:03Conclusion
Ein Verfahren für die menschliche induzierte pluripotente Stammzellen (IPSCs) über Retrovirus-vermittelte ektopische Expression von Oct4 generieren, wird Sox2, Klf4 und MYC beschrieben. Ein praktischer Weg, um menschliche iPSC Kolonien auf GFP-Expression zu identifizieren Basis wird ebenfalls diskutiert.
Tags
ReprogrammingHuman Somatic CellsInduced Pluripotent Stem CellsRetroviral VectorGFPHuman Embryonic Stem CellsPluripotentIn Vitro Disease ModelingRegenerative MedicineTranscription FactorsOct4Sox2Klf4MycAutologous CellsFibroblast CellsGFP-containing Retroviral BackboneESC Culture ConditionMorphologyRetroviral Transgene SilencingFluorescence MicroscopePluripotency GenesSurface Markers
