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Chapters
- 00:05Title
- 01:39Titration of Mutagen
- 05:59Enrichment of Egress Mutants
- 08:48Validation of Mutant Phenotypes by Egress Assays
- 10:21Cloning of Egress Mutants by Limiting Dilution
- 12:41Representative Data
- 14:14Conclusion
Forward genetica is een krachtige methode om moleculair niveau te ontrafelen van hoe<em> Toxoplasma</em> Egresses uit de gastheercel. Protocollen worden verstrekt aan chemisch mutageniseren parasieten, te verrijken voor mutanten met defecten in geïnduceerde uitgang en valideren het fenotype van gekloonde mutanten.
Tags
Genetic ScreenToxoplasma GondiiHost-cell Egress MutantsObligate Intracellular ParasiteLytic Replication CycleActive InvasionReplication Inside Host CellActive EgressMolecular LevelSignaling PathwaysPharmacological AgentsTriggers Of EgressIntracellular Ca2+Candidate Gene ApproachPlant Like Calcium Dependent Protein Kinase (CDPK)(chemical) Genetic ApproachesScreen For Mutant ParasitesDefect In Host Cell EgressChemical MutagenesisN-ethyl-N-nitrosourea (ENU)
