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测量人体血浆中的凝血因子V活性，用酶联凝固分析

JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

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测量人体血浆中的凝血因子V活性，用酶联凝固分析

DOI:

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13:08 min

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September 09, 2012

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Derek Tilley, Irina Levit, John A. Samis³

¹Applied Bioscience Program, Faculty of Science,University of Ontario Institute of Technology , ²Nursing Program, Faculty of Health Sciences,University of Ontario Institute of Technology , ³Medical Laboratory Science Program, Faculty of Health Sciences,University of Ontario Institute of Technology

Chapters

00:05Title
03:07FV 1-Stage Microplate Activity Assay
09:27FV 2-Stage Microplate Activity Assay
11:31FV Activity in Normal Human Plasma and in Patients With Disseminated Intravascular Coagulation (DIC)
12:15Conclusion

Summary

자동 번역

本研究描述了一种新颖的微孔板法测量FV凝血活性在人血浆中的纤维蛋白凝块的形成还没有被以前的报告。该方法使用一个动能酶标仪人血浆中的纤维蛋白凝块的形成过程中，连续地测量在405nm处的吸光度变化。

Tags

Factor V Activity Human Plasma Microplate Coagulation Assay Blood Coagulation Thrombin Fibrinogen Clot Formation Prothrombinase FV Assays Automated FV Assays Microplate Platform Enzyme-catalyzed Events Clot Lysis Platelet Aggregation Coagulation Factors FV Activity Measurement Kinetic Microplate Reader Absorbance Change At 405nm

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