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使用RIP-芯片的核糖核蛋白复合物的基因，小分子RNA和蛋白质组分的分离与鉴定细胞提取物的方法
JoVE 신문
생물학
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JoVE 신문 생물학
Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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使用RIP-芯片的核糖核蛋白复合物的基因，小分子RNA和蛋白质组分的分离与鉴定细胞提取物的方法

DOI:
10.3791/3851-v

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13:34 min

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September 29, 2012

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, , , , ,
1Molecular Microbiology and Immunology,University of Missouri, 2Department of Surgery,University of Missouri, 3Child Health,University of Missouri

Chapters

  • 00:00Title
  • 01:43Harvesting RNP Complex
  • 03:59Preparation of Isolation Materials
  • 06:40Immunoprecipitation
  • 08:42DNase and Proteinase K Treatment and RNA Precipitation
  • 12:11Representative Results
  • 12:49Conclusions

Summary

자동 번역

자동 번역

一步一步协议通过RIP-芯片的分离和鉴定RNA相关的复合物。

Tags

IsolationIdentificationMRNAsMicroRNAsProtein ComponentsRibonucleoprotein ComplexesRIP-ChipHigh-throughput SequencingMicroarray AnalysisGlobal Gene Expression AnalysisPost-transcriptional Gene RegulationRNA Binding Proteins (RBP)MRNA LocalizationStabilityTranslationRibonucleoprotein (RNP) ComplexDe Novo MRNA TargetsCellular ExtractsProtein OutputRNP Immunoprecipitation-microarray (RIP-Chip)Experimental ConditionsPost-transcriptional Gene Regulation MechanismsRibonucleoprotein Interactions

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