Journal
/
의학
/
Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)
JoVE 신문
의학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 의학
Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

Preparation of Myeloid Derived Suppressor Cells (MDSC) from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting (AutoMACS)

DOI:
10.3791/3875-v

•

14:15 min

•

June 18, 2012

•

, , ,
1Department of Molecular Medicine,University of South Florida Morsani College of Medicine

Chapters

  • 00:05Title
  • 03:11Generation of a Single-cell Suspension of Leukocytes from Spleens
  • 05:09Cell Surface Staining/Immunophenotyping of MDSC using Flow Cytometry
  • 09:01Magnetic Enrichment of Gr-1+ Leukocytes
  • 12:06Post-sort Analysis of Gr-1-enriched Leukocytes
  • 12:51AutoMACS Enrichment of Naive Gr-1+ Leukocytes for MDSC FACS Sorting
  • 13:40Conclusion

Summary

자동 번역

자동 번역

This is a rapid and comprehensive method of immunophenotyping Myeloid Derived Suppressor Cells (MDSC) and enriching Gr-1+ leukocytes from mouse spleens. This method uses flow cytometry and AutoMACS Cell Sorting to enrich for viable Gr-1+ leukocytes prior to FACS sorting of MDSC for use in vivo and in vitro assays.

Tags

Myeloid Derived Suppressor Cells (MDSC)Naive MicePancreatic Tumor-bearing MiceFlow CytometryAutomated Magnetic Activated Cell Sorting (AutoMACS)Heterogeneous PopulationImmature MacrophagesDendritic CellsGranulocytesLymphoid OrgansParasitic InfectionInflammationTraumatic StressGraft-versus-host DiseaseDiabetesCancerMac-1 (CD11b)Gr-1 (Ly6G And Ly6C)Tumor-bearing HostsAnti-tumor Immune ResponsesSubpopulations Of MDSCMolecular Mechanisms Of SuppressionGhansah GroupMurine Pancreatic Cancer ModelHomeostasisSuppressive FunctionLymphoid CompartmentsGr-1+ Leukocytes

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/3875?article_type=v

Magnetic Resonance Derived Myocardial Strain Assessment Using Feature Tracking
check_url/kr/3875?article_type=v

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
check_url/kr/3875?article_type=v

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
check_url/kr/3875?article_type=v

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
check_url/kr/3875?article_type=v

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
check_url/kr/3875?article_type=v

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications
check_url/kr/3875?article_type=v

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
check_url/kr/3875?article_type=v

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
check_url/kr/3875?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/kr/3875?article_type=v

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism (Microcystis aeruginosa) Using Flow Cytometry

Read Article