JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 03:11Generation of a Single-cell Suspension of Leukocytes from Spleens
- 05:09Cell Surface Staining/Immunophenotyping of MDSC using Flow Cytometry
- 09:01Magnetic Enrichment of Gr-1+ Leukocytes
- 12:06Post-sort Analysis of Gr-1-enriched Leukocytes
- 12:51AutoMACS Enrichment of Naive Gr-1+ Leukocytes for MDSC FACS Sorting
- 13:40Conclusion
هذا هو أسلوب سريع وشامل للالمناعي الظاهري متعلق بالنخاع الشوكي الخلايا المشتقة المكثف (MDSC) وإثراء GR-1<sup> +</sup> الكريات البيض من الطحال الماوس. هذا الأسلوب يستخدم التدفق الخلوي والخلية AutoMACS الفرز لإثراء لقابلة للحياة 1 غرام<sup> +</sup> الكريات البيض قبل FACS فرز MDSC للاستخدام<em> في الجسم الحي</em> و<em> في المختبر</em> المقايسات.
Tags
Myeloid Derived Suppressor Cells (MDSC)Naive MicePancreatic Tumor-bearing MiceFlow CytometryAutomated Magnetic Activated Cell Sorting (AutoMACS)Heterogeneous PopulationImmature MacrophagesDendritic CellsGranulocytesLymphoid OrgansParasitic InfectionInflammationTraumatic StressGraft-versus-host DiseaseDiabetesCancerMac-1 (CD11b)Gr-1 (Ly6G And Ly6C)Tumor-bearing HostsAnti-tumor Immune ResponsesSubpopulations Of MDSCMolecular Mechanisms Of SuppressionGhansah GroupMurine Pancreatic Cancer ModelHomeostasisSuppressive FunctionLymphoid CompartmentsGr-1+ Leukocytes
