JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Microbiology and Pathogenesis Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases,Centers for Disease Control and Prevention, 2Tick-Borne Diseases Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases,Centers for Disease Control and Prevention
Chapters
- 00:05Title
- 02:02Hemolymph Collection for Slide Preparation
- 03:11Salivary Gland Removal
- 04:38Saliva Collection
- 07:06Representative Results
- 07:52Conclusion
Indsamlingen af inficeret skovflåt hæmolymfe, spytkirtler, og spyt er vigtigt at undersøge, hvordan skovflåtbåren patogener forårsage sygdom. I denne protokol vi vise, hvordan man indsamler hæmolymfe og spytkirtler fra fodring<em> Ixodes scapularis</em> Nymfer. Vi har også demonstrere spyt samling af kvindelige<em> I. scapularis</em> Voksne.
Tags
SalivaSalivary GlandHemolymph CollectionIxodes Scapularis TicksTick-borne IllnessesLyme DiseaseTick-borne Relapsing FeverRocky Mountain Spotted FeverEhrlichiosisAnaplasmosisEncephalitisBabesiosisColorado Tick FeverTularemiaBorrelia Spp.Rickettsia RickettsiiEhrlichia ChaffeensisE. EquiAnaplasma PhagocytophilumTick-borne Encephalitis VirusBabesia Spp.ColtivirusFrancisella TularensisGene Expression RegulationTick Proteins InteractionTick MigrationBorrelia BurgdorferiEnzootic CycleHemostatic ResponseInnate Immune ResponseTick Saliva Proteins
